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Research Papers

Fabrication of a Cell Culture Plate With a Three-Dimensional Printed Mold and Thermal Analysis of PDMS-Based Casting Process

[+] Author and Article Information
Myo Min Zaw

Department of Mechanical Engineering,
University of Maryland Baltimore,
County 1000 Hilltop Circle,
Baltimore, MD 21250
e-mail: myo1@umbc.edu

William D. Hedrich

Department of Pharmaceutical Sciences,
University of Maryland School of Pharmacy,
Baltimore, MD 21201
e-mail: whedrich@umaryland.edu

Timothy Munuhe

Mem. ASME
Department of Mechanical Engineering,
University of Maryland Baltimore,
County 1000 Hilltop Circle,
Baltimore, MD 21250
e-mail: tim.munuhe@umbc.edu

Mohamad Hossein Banazadeh

Department of Mechanical Engineering,
University of Maryland Baltimore,
County 1000 Hilltop Circle,
Baltimore, MD 21250
e-mail: mh.banazade@gmail.com

Hongbing Wang

Department of Pharmaceutical Sciences,
University of Maryland School of Pharmacy,
Baltimore, MD 21201
e-mail: hwang@rx.umaryland.edu

S. Andrew Gadsden

Mem. ASME
College of Engineering and Physical Sciences,
University of Guelph,
Guelph, ON N1G 2W1, Canada
e-mail: gadsden@umbc.edu

Liang Zhu

Mem. ASME
Department of Mechanical Engineering,
University of Maryland Baltimore,
County 1000 Hilltop Circle,
Baltimore, MD 21250
e-mail: zliang@umbc.edu

Ronghui Ma

Mem. ASME
Department of Mechanical Engineering,
University of Maryland Baltimore,
County 1000 Hilltop Circle,
Baltimore, MD 21250
e-mail: roma@umbc.edu

Contributed by the Heat Transfer Division of ASME for publication in the JOURNAL OF THERMAL SCIENCE AND ENGINEERING APPLICATIONS. Manuscript received October 17, 2017; final manuscript received April 4, 2018; published online July 13, 2018. Assoc. Editor: Samuel Sami.

J. Thermal Sci. Eng. Appl 10(6), 061002 (Jul 13, 2018) (8 pages) Paper No: TSEA-17-1398; doi: 10.1115/1.4040134 History: Received October 17, 2017; Revised April 04, 2018

Polydimethylsiloxane (PDMS)-based casting method was used to fabricate PDMS cell culture platforms with molds printed by a fused deposition modeling (FDM) printer. Cell viability study indicated that the produced plates have the suitable biocompatibility, surface properties, and transparency for cell culture purposes. The molds printed from acrylonitrile-butadiene-syrene (ABS) were reusable after curing at 65 °C, but were damaged at 75 °C. To understand thermal damage to the mold at elevated temperatures, the temperature distribution in an ABS mold during the curing process was predicted using a model that considers conduction, convection, and radiation in the oven. The simulated temperature distribution was consistent with the observed mold deformation. As the maximum temperature difference in the mold did not change appreciably with the curing temperature, we consider that the thermal damage is due to the porous structure that increases the thermal expansion coefficient of the printed material. Our study demonstrated that FDM, an affordable and accessible three-dimensional (3D) printer, has great potential for rapid prototyping of custom-designed cell culture devices for biomedical research.

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Figures

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Fig. 1

(a) PDMS culture plate design and (b) mold with negative pattern

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Fig. 2

Schematic of curing process in the oven

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Fig. 3

(a) A Petri dish filled with PDMS liquid after degasification and (b) a final PDMS platform after curing

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Fig. 4

A damaged ABS mold after curing for an hour at 75 °C

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Fig. 5

(a) Surface temperature of the ABS mold and (b) temperature distribution on the cross-sectional plane N–N after curing at 65 °C for 60 min

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Fig. 6

(a) Surface temperature of the ABS mold and (b) temperature distribution on the cross-sectional plane N–N after curing at 75 °C for 60 min

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Fig. 7

Simulated surface temperature of the PDMS after curing at (a) 65 °C and (b) 75 °C for 60 min

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Fig. 8

The temporal evolution of the highest and the lowest temperature in the ABS mold during the curing process

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Fig. 9

(a) Cells cultured in the commercial 12 well plate and (b) in PDMS plates after 72 h

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Fig. 10

Comparison of cell viability cultured in 12-well plate and PDMS plates

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