Low Reynolds number laminar channel flow is used in various heat/mass transfer applications such as cooling and mixing. A low Reynolds number implies a low intensity of heat/mass transfer processes since they rely only on the gradient diffusion. To enhance these processes, an active flow control by means of synthetic (zero-net-mass-flux) jets is proposed. The present study is experimental, in which a Reynolds number range of 200–500 is investigated. Measurement has been performed mainly in air as the working fluid by means of hot-wire anemometry and the naphthalene sublimation technique. Particle image velocimetry (PIV) experiments in water are also discussed. The experiments have been performed in macroscale at the channel cross sections and in air and water, respectively. The results show that the low Reynolds number channel flow can be influenced by an array of synthetic jets. The effect of synthetic jets on the heat transfer enhancement is quantified. The stagnation Nusselt number is enhanced by 10–30 times in comparison with the nonactuated channel flow. The results indicate that the present arrangement can be a useful tool for heat transfer enhancement in various applications, e.g., cooling and mixing.